Lamin A/C anticorps

N° du produit ABIN126715

Cet anticorps Souris Monoclonal détecte spécifiquement Lamin A/C dans WB, IF et IHC (fro). Il présente une réactivité envers Humain, Boeuf (Vache), Xenopus laevis et Poisson.

Aperçu rapide pour Lamin A/C anticorps (ABIN126715)

Antigène: Lamin A/C (LMNA)

Reactivité: Humain, Boeuf (Vache), Xenopus laevis, Poisson

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Lamin A/C est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: X67

Détail du produit anti-Lamin A/C anticorps

Specificité: The monoclonal antibody cocktail decorates the karyoskeleton, i.e. the intermediate filament equivalent of the nucleus. Polypeptides reacting: Lamin isotypes of Mr 60 - 75 kDa.

Purification: Protein A affinity chromatography

Immunogène: Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells)

Isotype: IgG1

Information d'application

Indications d'application: Western blotting. Immunofluorescence microscopy. Immunohistochemistry: dilute further 1: 10 (incubation time 1 h at room temperature).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in 1.0 mL distilled water.

Buffer: 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Lamin A/C

Antigène: Lamin A/C (LMNA)

Autre désignation: Lamin-A/C (LMNA)

Sujet: Nuclear Lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type Lamins and B-type Lamins. The A-type Lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. Lamin A, Lamin C and Lamin Adel 10, while the B-type Lamins include two proteins arising from two distinct genes, i.e. Lamin B1 and Lamin B2. Recent evidence has revealed that mutations in A-type Lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type Lamins coincides with cell differentiation and as A-type Lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type Lamins.Synonyms: 70 kDa Lamin, LMN1, LMNA, Lamin A, Lamin A + C, Lamin-A/C, NY-REN-32, NYREN32, Nuclear Envelope Marker, Renal carcinoma antigen NY-REN-32

ID gène: 4000

NCBI Accession: NP_005563

UniProt: P02545

Pathways: Apoptose, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus